Synthesis of nanomaterials for efficiently detecting pharmaceutical medicaments such as paracetamol (PRC) is important for industrial and pharmacological applications. In this study, we prepared copper oxide nanospheres (CuO-NSPs) via facile chemical co-precipitation along with the sonochemical technique. The prepared CuO-NSPs were fabricated on a disposable screen-printed carbon electrode (SPCE) utilized for conducting sensitive and selective electrochemical detection of PRC. The CuO-NSPs were systematically characterized using various spectroscopic techniques, including X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive X-ray analysis, field emission scanning electron microscopy, and Raman spectra. The electrochemical properties of CuO-NSPs were verified using electrochemical impedance spectroscopy (EIS), Cyclic voltammetry (CV) and linear sweep voltammetry techniques. As an electrochemical sensor, the proposed sphere-like copper oxide screen printed carbon electrode (CuO-NSPs/SPCE) demonstrated an excellent sensitivity with a wider linear response range from 0.01 to 1480µM. The electrode also has good sensitivity. Different coexisting compounds were present when the selectivity was tested. Last but not least, the CuO-NSPs/SPCE consistently demonstrates reproducibility, durability, and other critical aspects for future development as a point-of-care device.